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β-fructofuranosidases (FFases) are enzymes involved in sucrose hydrolysis and fructo-oligosaccharides’ production which are of great interest for the food industry. FFase from  Aspergillus tamarii  URM4634 was extracted using PEG/Phosphate Aqueous Biphasic Systems (ABS), and the impact of magnetic field on the extraction behavior was evaluated. A 2 4 -full experimental design was employed to study the influence of molar mass of PEG, concentrations of PEG and phosphate and pH on the selected response variables, i.e., partition coefficient ( K ), purification factor ( PF ), activity yield ( Y ) and selectivity ( S ). The influence of magnetic field during partition and NaCl concentration on the same responses was also studied. The best results of FFase extraction without magnetic field ( K  = 0.50,  PF  = 4.05,  Y  = 72.66% and  S  = 0.06) were observed at pH 8.0 using 12.5% (w/w) PEG 400 and 25% (w/w) NaH 2 PO 4 /K 2 HPO 4 . Application of the magnetic field allowed improving the performance, with the best results being obtained at the longest distance between magnets (lowest magnetic field) and absence of NaCl ( K  = 0.93,  PF  = 4.22,  Y  = 83.79% and  S  = 0.09). The outcomes obtained demonstrate that ABS combination with low intensity magnetic field can be used as an efficient FFase pre-purification method.

preparative biochemistry and biotechnology

Extraction and purification of  Aspergillus tamarii  β-fructofuranosidase with transfructosylating activity using aqueous biphasic systems (PEG/phosphate) and magnetic field