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The lack of a viable vaccine and the emergence of novel  Mycobacterium tuberculosis  (MTB) strains that are particularly resistant to treatments, presage a complicated future situation. Biosynthesized nanomaterials are currently proving to be a viable antibacterial therapeutic option, including for MTB infection treatment. The goal of this work is to synthesize silver nanoparticles (AgNPs) from  Syzygium aromaticum  seeds and investigate their antimicrobial, anti-tubercular, and cytotoxic properties using zebrafish embryos and  Artemia salina . The UV spectrophotometer, SEM-EDAX, TEM and FTIR measurements were used to characterize the AgNPs. Antibacterial activity was performed against  S. aureus ,  P. aeruginosa ,  E. coli  and  K. pneumoniae  and exhibited potential inhibitory activity towards the bacterial cultures. The MABA assay was used to investigate the anti-mycobacterial activity, and the AgNPs showed the highest percentage of inhibition in both test concentrations (250 and 500  µg/ml).

materials technology

Green synthesis of biofabricated silver nanoparticles from  Syzygium aromaticum  seeds: spectral characterization and evaluation of its anti-mycobacterial activity, cytotoxicity assessment on zebrafish embryo and  Artemia salina