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Seawater based bio-cementation through microbially-induced carbonate precipitation was proposed for the calcareous sand improvement in marine environment. The method used seawater instead of traditional fresh water to culture urease producing-bacteria (UPB) and prepare cementation solution (CS) for the bio-cement. A series of comparative bio-treatment tests using seawater based bio-cementation and traditional bio-cementation methods on three types of soil were conducted. Experimental results indicate that seawater based bio-cementation method has the ability to improve soil physico-mechanical properties, and performed better than traditional bio-cementation method. The dominant reason can be explained as that the mixture of the productions of calcite, monohydrocalcite and calcite magnesium produced during seawater based bio-cementation process have better cementation ability than the mixture of the productions of calcite and vaterite produced during bio-cementation process. UCS of coarse Ottawa sand blocks are smaller than that of medium Ottawa sand blocks is because the specific surface area of fine sand is higher and larger number of effective bondings can be formed. UCS of calcareous sand blocks are smaller than those of coarse Ottawa sand blocks can be attributed to the fact that calcareous sand has higher porosity and rougher surface, resulting in more carbonate crystals being precipitated on un-connected locations.

Seawater based bio-cementation for calcareous sand improvement in marine environment