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By comparing the cytochrome b fragment sequences of sablefish and 16 varieties susceptible to adulteration, a real-time polymerase chain reaction (PCR) method was established to detect sablefish-derived components. The PCR method was used to analyze commercial products. The method developed was highly specific without any cross-reaction in the other 16 species and with a limit of detection for deoxyribonucleic acid (DNA) of 0.002 ng µL −1  for sablefish. In addition, the mixed muscle tissues were detected at as low as 0.01 g kg −1 . The applicability of the PCR method to 50 commercial prepackaged products revealed the accuracy of this method.

journal of aquatic food product technology

Development and Evaluation of Real-Time Polymerase Chain Reaction (PCR) to Authenticate Sablefish ( Anoplopoma fimbria ) in Commercial Seafood Products