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This study assesses the nutritional aspects of five  Gracilaria  sp. ( G. verrucosa, G. corticata, G. crassa, G. cylindrical , and  G. edulis ) including their antioxidant and  in-vitro  anti-diabetic potential. Liquid chromatography-mass spectrometry (LC-MS) reveals the presence of free amino acids. Total phenolic content 13.25 ± 0.39 mg GAE g-1, flavonoid content 44.45 ± 0.36 mg GAE g-1, ferrous ion chelating ability 28.32%, and hydrogen peroxide radical scavenging activity 16.53 ± 0.5% contents were recorded highest in  G. crassa , while DPPH activity was recorded highest in  G. corticata  33.7 ± 0.35%, and total polyamine content was recorded maximum in  G. edulis  (2.54 ± 0.27). Polysaccharide yield in red alga  Gracilaria  sp. varies from highest (25.14 ± 0.6) in  G. crassa  to lowest (16.99 ± 0.53) in  G. edulis . The highest number was 12 types of free amino acids present in  G. edulis . The highest α‑amylase (46.80%) was recorded in  G. crassa , while the highest α‑glucosidase (80.63%) was observed in  G. corticata .

Assessment of Nutritional Constituents Content and Biomedical Aspects of Five Gracilaria Species: A Multivariate Analysis