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A rapid and efficient micropropagation protocol was developed for  Curcuma zedoaria , using shoot buds as explants. Explants established on basal MS medium were transferred to 12 different medium compositions with different combinations and concentrations of plant growth hormones 6-benzylaminopurine (BAP), kinetin (Kn), and thidiazuron (TDZ). The best shoot multiplication was recorded in MS medium supplemented with BAP (2.0 mg L −1 ) and TDZ (1.5 mg L −1 ), resulting in 5.3 ± 0.24 shoots of per explant and shoot length of 6.12 ± 0.19 cm. For the best  in vitro  root induction, the aseptic shoots excised from the culture flasks and inoculated individually on MS medium supplemented with 1.5 mg L −1  indole-3-butyric acid (IBA) resulted in 74.2% root growth and 8.82 ± 0.31 roots per explant. Although callus induction was observed in MS containing 2,4-dichlorophenoxyacetic acid (2, 4-D) and 1-naphthaleneacetic acid (NAA), the highest callus proliferation was observed with MS and NAA (2.0 mg L −1 ). Micropropagated plants established on soil showed 83% survival on acclimatization to the field conditions.

High-frequency Shoot Multiplication and In Vitro Plantlet Regeneration from Shoot Bud Explants in Curcuma zedoaria Roscoe