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Flaxseed essential oil extracted by hydrodistillation was analyzed for its anticancer effects on A549 lung cancer cells. Phytochemicals were analyzed using Fourier-transform infrared spectroscopy (FTIR) and Gas Chromatography-Mass Spectrometry (GC-MS). The cytotoxicity effect was analyzed using Trypan blue, MTT, Clonogenic, DNA fragmentation, and wound scratch assays. Seventeen compounds with anticancer potency were identified. The cytotoxicity increased with increasing concentrations and inhibited migration of the cancer cells. Smeared DNA in gel and increased OD values compared to intact control DNA were also possible because of the phytochemicals in the essential oils.

journal of herbs, spices and medicinal plants

The Phytochemicals of  Linum usitatissimum  Seed Essential Oil Extracted by Hydrodistillation Induced Apoptosis and Suppressed Cell Migration in A549 Lung Cancer Cells