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Micropropagation protocol for  Diplocyclos palmatus  through direct shoot induction from isolated cotyledon and leaf explants was established. Cotyledons were isolated from 2-week-old seedlings and cultured in the Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP, 0.5–2.0 mg L −1 ) alone or in combination with α-naphthalene acetic acid (NAA, 0.2–1.0 mg L −1 ) for shoot induction. The highest frequency of response (89%) and the mean number of shots (6.8) were observed in an MS medium supplemented with 1.5 mg L −1  BAP and 0.5 mg L −1  NAA. Leaves at various stages of development were isolated from  in vitro  grown shoots and cultured in an MS medium supplemented with different concentrations of BAP (0.5–2.5 mg L −1 ), Thidiazuron (TDZ, 0.5–2.5 mg L −1 ) alone and in combination with NAA (0.2 mg L −1 ) for shoot induction. The highest shoot induction frequency of 91% and shoot number of 7.8 shoots per explant were observed when 15-d-old- leaves were cultured in an MS medium enriched with 2.0 mg L −1  TDZ and 0.5 mg L −1  NAA after 45 d. The isolated cotyledon and leaf-derived shoots showed optimum rooting on ½ MS medium fortified with 4.0 mg L −1  indole-3-butyric acid (IBA). The rooted plants were transferred to the soil with 90% success.

In Vitro Plant Regeneration via Adventitious Shoot Regeneration from Cotyledon and Leaf Explants of Diplocyclos Palmatus