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Phytochemicals in the ethanol extract of  Artemisia herba-alba  were examined and evaluated for the antioxidant and cytotoxicity potential. The extraction was performed using an accelerated solvent extraction (ASE300), analyzed by GC-MS and the total phenolics was estimated by Folin–Ciocalteau method. The antioxidant activity of the extract was evaluated using three methods and its cytotoxicity was evaluated  in-vitro  on NCI-N417 tumor cell line using MTT test and flow cytometry. Twenty-one compounds were identified in the  A. herba-alba  extract, which was rich in phenolic compounds (248.56 ± 11.05 μg GAE mg −1  extract) and had antioxidant activity characterized by a high DPPH-radical-scavenging activity (IC50 = 69.01 ± 0.03 μg mL −1 ), inhibitory effect of β-carotene oxidation (95.75%±0.013), and reducing power (DO700nm = 0.751 ± 0.001). Ethanol extract from  A. herba-alba  demonstrated cytotoxic and antiproliferative activity.

Antioxidant Activity and Polyphenols Content of Artemisia Herba-Alba Extract and their Cytotoxicity against Human Lung Cancer Cells NCI-N417