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Gymnema  is a genus of the Apocynaceae with more than 50 species. Some of its species such as  G. sylvestre and G. latifolium  have been reported for their medicinal value. In this study, the genetic diversity of 20 samples of four species of  Gymnema  in Vietnam was evaluated using the Random Amplification of Polymorphic DNA (RAPD) and the internal transcribed spacer of nuclear ribosomal DNA (ITS-rDNA). Amplified bands obtained with primers UBC701, UBC709, and OPC4 were the highest polymorphic in RAPD analysis. The phylogenetic trees were built grouping the 20 samples into four clusters corresponding to the four  Gymnema  species according to morphological classification. A number of characteristic SNPs were identified for each species which can be used for authentication and differentiation. The results showed the consensus and usefulness of the two barcoding techniques in the molecular identification and characterization of the  Gymnema  genus.

Assessment of Genetic Variation of the Genus Gymnema in Vietnam, Using RAPD and ITS-rDNA Markers