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The flavonoids and phenolics contents, antioxidant potential, and enzymes ( α- amylase, lipase, elastase, tyrosinase, acetylcholinesterase, and butyrylcholinesterase) inhibition potential of leaf extracts from  Elaeocarpus sphaericus, Santalum album, Pterocarpus santalinus , and  Ziziphus budhensis  collected from Ramechhap District of Nepal were evaluated. DPPH assay was used to determine the antioxidant potential, and the DNSA method was used to examine  α- amylase inhibition activity. P-NPB, L-DOPA, AAAPVN, acetylthiocholine iodide, and butyrylthiocholine iodide were used as respective substrates for the determination of lipase, tyrosinase, elastase, acetylcholinesterase, and butyrylcholinesterase inhibition activities. The crude extract of  P. santalinus  showed inhibitory potential against all enzymes, whereas  E. sphaericus, S. album , and  Z. budhensis  extracts showed moderate inhibition activity. However,  S. album  did not inhibit lipase, elastase, and tyrosinase.

Antioxidant and Enzymes Inhibitory Activities of Leaf Extracts of Plant Species Traditionally Used for Medicinal and Spiritual Purposes in Nepal