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The study compared efficacy of three sperm selection techniques in improving freezability of low-quality Murrah buffalo bull ejaculates. Sephadex (SEP), Sephadex ion-exchange filtration (SIE), and 40/80%  BoviPure™(BP)  gradient centrifugation protocols were standardized (ejaculates,  n  =   24). In Experiment-I, Sephadex G-75, G-100, and combined Sephadex G (75–100) column filtrates were compared. In Experiment-II,  BP  protocols: 200   g-10   min, 250   g-5, and 10   min, 300   g-10, and 15   min were compared. In fresh semen, Sephadex G (75–100) filtration and 250   g-5   min  BP  protocol improved sperm functions and were used in Experiment-III, where SEP G (75–100), SIE G (75–100), and 250   g-5   min  BP  processed ejaculates ( n  =   48) were cryopreserved and compared at post-thaw stage. The mean recovery rate differed in order: SEP &gt; SIE &gt;  BP . SIE filtration significantly improved progressive motility, livability, membrane integrity, bovine cervical mucus penetration and live non-apoptotic sperm. Compared with control, all three techniques equally reduced post-dilution and post-thaw lipid peroxidation (LPO) rate. SEP post-thaw filtrates observed lower cryocapacitation-like changes, LPO (C 11 ‐BODIPY 581/591 ), and higher active mitochondria than other treatments. SIE and SEP equally improved post-thaw acrosome-intact sperm over  BP . Filtration techniques, preferably, Sephadex ion-exchange filtration can most efficiently process low-quality buffalo bull ejaculates for cryopreservation and improve freezability.

animal biotechnology

Filtration techniques are advantageous over colloidal centrifugation in improving freezability of low-quality buffalo bull ( Bubalus bubalis ) ejaculates