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AGPAT6 plays a crucial role in the triglyceride (TG) synthesis pathway in mammals. However, its roles in buffalo lactation remain unknown. Therefore, we investigated the functional roles of  AGPAT6  in milk fat synthesis by transfecting overexpression and lentivirus interference vectors in buffalo mammary epithelial cells (BuMECs) in vitro.  AGPAT6  overexpression in BuMECs significantly enhanced the mRNA expression of  FABP4 ,  SLC27A6, ACSL1 ,  DGAT1 ,  DGAT2 ,  LPIN1 ,  INSIG1 ,  CEBPA  and  SREBF1  genes, and significantly reduced that of  XDH ,  CPT1A ,  LIPE ,  INSIG2  and  PPARGC1A , but has no significant influence to the mRNA abundance of  FABP3 ,  GPAM, PPARG  and  SREBF2 . However, the interference with  AGPAT6 , the mRNA expression of  FABP4 ,  SLC27A6 ,  ACSL1 ,  DGAT1 ,  DGAT2 ,  INSIG1 ,  CEBPA, SREBF1 ,  XDH ,  CPT1A ,  LIPE ,  INSIG2  and  PPARGC1A  genes in BuMECs changed contrary to the overexpression experiment, and that of  GPAM ,  PPARG  and  SREBF2  also did not change significantly, but the expression of  FABP3  was significantly decreased. In addition, the overexpression/interference of  AGPAT6  gene significantly increased/decreased TG content in BuMECs. The results here indicate that  AGPAT6  gene is involved in TG synthesis in BuMECs, and affects the expression of major genes associated with FA transport and activation, TG synthesis and transcription regulation, FA oxidation and TG degradation during the lipogenesis of milk.

Functional roles for AGPAT6 in milk fat synthesis of buffalo mammary epithelial cells