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Spermatogenesis is the developmental process that produces spermatozoa. The aim of this study was to investigate the single nucleotide polymorphisms (SNPs) within  C7H15orf39  and  NOS2  genes and to determine the correlations between two SNPs and semen quality in Duroc boars ( n  = 604). The polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) method was used for genotyping the selected two nonsynonymous SNPs. The significant correlation was observed between two SNPs (rs80969873: g.58385473 G &gt; A within  C7H15orf39 ; rs325865291: g.44175445 G &gt; A within  NOS2 ) and semen traits in Duroc boars. This study indicates the SNPs in  C7H15orf39  and  NOS2  may be the potential molecular marker for improving the semen quality traits in Duroc boars.

Nonsynonymous SNPs within C7H15orf39 and NOS2 are associated with boar semen quality