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Aquaculture species are often exposed to acute stressors such as low water levels and handling during routine aquaculture procedures. This might result in oxidative stress by the increased reactive oxygen species (ROS)’ production ( e.g. , superoxide anion). The harmful effects of ROS are eliminated by a defense system, referred antioxidant defense system (ADS).  sod1  is the first gene involved in the ADS. Therefore, we cloned and characterized the open reading frame of the  sod1  in brown trout. Then, we determined the effects of low water level and handling stress on  sod1  mRNA expression in the liver and gills at 0 min, 1 and 2 h. The total RNA isolated was used to synthesize cDNA for RT-qPCR analysis. Phylogenetic tree, identity/similarity percentages, genomic organization, and conserved gene synteny analyses were applied to characterize Sod1/ sod1 . While low water level stress upregulated  sod1  expression in the liver compared to the control group, no significant differences were observed in the gills between experimental groups. However, brown trout differently responded to handling stress at different time intervals in both tissues. Transcriptional differences were also noted between the sexes. This study contributes to the current understanding of the molecular mechanism between oxidative stress and ADS.

animal biotechnology

Molecular cloning and characterization of Cu-Zn superoxide dismutase ( sod1 ) gene in brown trout and its expression in response to acute aquaculture stressors