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This study aimed to determine the influence of  in ovo  injection of  Astragalus membranaceus  polysaccharide on growth, feed consumption, feed conversion ratio, carcasses, hematology, and blood metabolites in Cobb 500 chicks. At the 7th day of incubation, a total of 250 eggs were randomly divided into five groups with 5 replications of 10 eggs of each: negative control (normal eggs), positive control (0.5 mL saline), 1.5 mg  Astragalus membranaceus , 3.0 mg  Astragalus membranaceus , and 4.5 mg  in ovo Astragalus membranaceus  injection. Live body weight and gain were not significantly (linear and quadratic) affected by  in ovo  injection of  Astragalus membranaceus . Kidney and liver functions were influenced by  in ovo  injection of  Astragalus membranaceus  polysaccharides in broiler chickens. Antioxidant enzymes were quadratically increased with  Astragalus membranaceus  polysaccharides, and the highest values achieved with 4.5 mg. The MDA concentration was linearly and quadratically decreased with  in ovo  injection of  Astragalus membranaceus  polysaccharides when compared to negative control. The highest values of IgG and IgM were achieved with 1.5 mg  Astragalus membranaceus  polysaccharides when compared to all other groups. In conclusion, our results indicate that  in ovo  injection of  Astragalus membranaceus  polysaccharides 1.5–4.5 mg in broiler eggs significantly improved serum ALT, AST, AP, creatinine enzymes, antioxidant activity, and immune function.

animal biotechnology

Early nutrition programming with  Astragalus membranaceus  polysaccharide: its effect on growth, carcasses, immunity, antioxidants, lipid profile and liver and kidney functions in broiler chickens