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Goose ( Anser cygnoides ) are highly broody with low egg production, and large number of granulosa cells undergo apoptosis during broodiness. Our previous study has found that  miR-34c-5p  was highly abundant in the ovary of goose with broodiness phenotype. However, the mechanism that  miR-34c-5p  regulates granulosa cells function remains unclear. Here, we demonstrated that broody goose had higher levels of  miR-34c-5p  than that in laying goose by qRT-PCR. The dual luciferase reporter assay showed that  Bcl2  was identified as a direct target of  miR-34c-5p , which could be negatively regulated by  miR-34c-5p . Furthermore, over-expression of  miR-34c-5p  significantly increased the rate of apoptosis and slowed down the proliferation of granulosa cells by inhibiting the  Bcl2  expression, whereas the opposite trend was obtained when granulosa cells were supplemented with  miR-34c-5p  inhibitors. In addition,  Bcl2  mRNA level was lower in goose with a brooding phenotype than that in goose with an egg-laying phenotype. Taken together, the data suggested that  miR-34c-5p  regulated granulosa cells apoptosis and brooding behavior by targeting  Bcl2 , which not only contribute to reveal the potential mechanism of  miR-34c-5p  underlying granulosa cells apoptosis in goose, but also provides an effective strategy to reduce the incidence of broodiness and improve the egg production.

MiR-34c-5p promotes granulosa cells apoptosis by targeting Bcl2 in broody goose ( Anser cygnoides )