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A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation.  Brucella abortus ,  Leptospira interrogans  serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were mixed in bovine semen samples. The µcLAMP assay is portable, user-friendly, cost-effective, and suitable to be performed as a POC diagnostic test. We have demonstrated high sensitivity and specificity of µcLAMP for detection of  Brucella ,  Leptospira,  and BoHV-1 in bovine semen samples comparable to PCR and qPCR assays. Thus, µcLAMP would be a promising field-based test for monitoring various infectious pathogens in biological samples.HighlightsDetect infectious organism in bovines semenReduction in carryover contamination is an important attribute, which may reduce the false-positive reaction.µcLAMP is a miniaturized form, which could be performed with a minimum volume of reagents.The µcLAMP assay is portable, user-friendly, and suitable to be performed as a POC diagnostic test. Detect infectious organism in bovines semen Reduction in carryover contamination is an important attribute, which may reduce the false-positive reaction. µcLAMP is a miniaturized form, which could be performed with a minimum volume of reagents. The µcLAMP assay is portable, user-friendly, and suitable to be performed as a POC diagnostic test.

Microcapillary LAMP for rapid and sensitive detection of pathogen in bovine semen