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Jewel tetra ( Hyphessobrycon eques ) is a freshwater fish found in several rivers and basins in South America. The present study is the first study to create a panel of microsatellite markers for detecting genetic diversity in  H. eques  and evaluating the application of these markers in  Serrapinnus notomelas . In total, 44 individuals were genotyped from the natural (WIL,  n  = 20) and stock in captivity (CAP,  n  = 24) population. Moreover, 19 microsatellite markers were obtained, of which only 8 loci presented a high degree polymorphism. In total, 45 alleles were detected, ranging from 126 bp (Hype2G2) to 420 bp (Hype2E2). The Hardy–Weinberg equilibrium ( p  &lt; 0.05) revealed significant difference in one locus in WIL (Hype1G4) and three loci in CAP (Hype1F4, Hype2C3, and Hype2G2). Null alleles ( p  &lt; 0.05) were present in only one locus (Hype1G4). The WIL and CAP populations revealed high genetic diversity during F ST  analysis. The cross-amplification test for  S. notomelas  revealed that only two loci (Hype2C3 and Hype2G2B) presented satisfactory transferability results. The developed microsatellite primers will be useful in studying the genetic diversity and population structure of  H. eques  in wild populations and fish farms in the Brazilian and other South American basins.

animal biotechnology

Evaluation of a panel of microsatellite markers to study their applications in  Serrapinnus notomelas  and to reveal the genetic diversity in  Hyphessobrycon eques