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This study investigated the effects of dietary mixed and sprayed of  Bacillus subtilis  subsp.  inaquosorum  IS02 ( B.s.i . IS02) on growth performance and digestive enzyme activity of whiteleg shrimp. Three different dosages of  B.s.i . IS02 were applied via two application methods: mixed with (TM) and sprayed on (TS) feed containing 10 8  (TM1 and TS1), 10 7  (TM2 and TS2), 10 6  (TM3 and TS3) CFU Kg −1 . Compared to control groups, growth performance was significantly higher in treated shrimp. There was a significant difference in survival rate between TM1 (94.44 ± 1.57%) and TM2 (87.78 ± 1.92%) compared to the other diets. Furthermore, the activity of digestive enzymes was significantly higher in TS1. Overall, it can be concluded that spraying  B.s.i . IS02 at the dosage of 10 7  or 10 8  CFU kg −1  on feed can be a useful method in the farm; also, mixing at the dose of 10 8  CFU kg −1  may prove effective in feed factories.

journal of applied aquaculture

Dietary mixed and sprayed probiotic improves growth performance and digestive enzymes of juvenile whiteleg shrimp ( Litopenaeus vannamei , Boone, 1931)