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The nutritional content of  P. pacifica  for protein ranges from 40% to 50% fat 10–15% and EPA is 7.52% not much different from the nutritional content of  Artemia sp . i.e. 45–55%, fat 10–12%. The aim of the study was to find of the effect of different levels of substrate ( C. lentillifera ) in the culture of  P. pacifica  resulting in the best growth performance and nutritional quality of  L. vannamei . The  L. vannamei  used, weighing 0.029 ± 0.001 g were stocked at a density of 5 individual L-1 and reared for 30 days. Treatment of Pacific white shrimp larvae feeding with  P. pacifica  resulted from mass culture using a substrate the  P. pacifica  cultured at different levels of  C. lentillifera  as a substrate: A (0 g m −2 ); B (20 g m −2 ); C (40 g m −2 ); and D (60 g m −2 ). The results showed that the best treatment of pacific white shrimp fed by  P. pacifica  cultured using substrate  C. lentillifera  60 g m −2  (D). The results showed that post-larvae of  L. vannamei  shrimp fed by  P. pacifica  cultured using the substrate of  C. lentillifera  60 gram/m 2  (D) produces the highest value on RGR of 34.98%, the absolute length growth of 1.97 cm; biomass weight of 0.0104 g; the survival rate of 96% and grazing rate 23.034 individual day −1 . 62.15% of protein and 8.04% of fat; the EPA was 7.95%, and lysine was 11.95%.

journal of applied aquaculture

The effect of mass cultured  Phronima pacifica  feed using  Caulerpa lentillifera  substrate on growth performance and nutritional quality of pacific white shrimp ( Litopenaeus vannamei )