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Male buffalo ( Bubalus bubalis ) calves (n = 10) were divided equally into control (fed basal diet had 0.23 ppm Se) and treatment (fed additional supra-nutritional Se at 0.3 mg/kg dry matter intake) groups to evaluate the nutraceutical role of selenium. Nutraceutical level of Se enhanced (P &lt; 0.01) the percentage of monocyte cells in blood as well as Se level of plasma, without influencing most of the studied hemotological parameters including macro and micro minerals. Among oxidative stress markers, lipid peroxidation level was low (P &lt; 0.05) in the treatment group at the end of the feeding trial with a high (P &lt; 0.05) overall average of the antioxidative enzyme glutathione peroxidase. Parameters like reduced glutathione, catalase, superoxide dismutase, ceruloplasmin, vitamins A, E, and β-carotene remained unaltered despite variable levels of Se in the diet. Cellular immune response against bacteria  P. multocida  remained uninfluenced (P &gt; 0.05), though an enhanced (P &lt; 0.01) average antibody titer was noted in the group fed nutraceutical level of Se. Findings of the study suggest that supra-nutritional Se (0.53 ppm against 0.23 ppm) had a nutraceutical role and thus favoring humoral immune response, antioxidant status, blood monocyte percentage, and plasma Se levels in the healthy buffalo calves.

Nutraceutical role of supra-nutritional selenium in healthy buffalo ( Bubalus bubalis ) calves