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This study analyzed the chemical composition of OGEO and its effects on growth, viability, antrum formation and mRNA expression for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and peroxiredoxin 6 (Prdx6) in bovine secondary follicles cultured  in vitro . The follicles were cultured in TCM-199+ alone or supplemented with 0.125, 0.25 and 0.50% OGEO for 18 days. Follicle diameters, viability, antrum formation and the levels of mRNA for  SOD, CAT, GPX1  and  PRDX6  in cultured follicles  were evaluated. The results showed that the main components of OGEO were Eugenol and 1.8-Cineol. The follicles cultured with OGEO maintained morphology, viability and growth similar to the control group. Interestingly, follicles cultured with 0.5% or 0.25% OGEO had, respectively, higher levels of mRNAs for PRDX6 and CAT than those cultured in control medium. In conclusion, OGEO increases mRNA expression for PRDX6 and CAT in bovine secondary follicles cultured  in vitro .

journal of essential oil research

Chemical composition and effects of  Ocimum gratissimum  essential oil (OGEO) on the expression of mRNA for antioxidant enzymes during  in vitro  culture of bovine ovarian secondary follicles