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Bovine Serum Albumin (BSA) was used as a green, inexpensive and efficient biocatalyst, for synthesis of 1 H -pyrazolo[1,2- b ]phthalazine-5,10-dione and 1 H -pyrazolo[1,2-a]pyridazine-5,8-dione derivatives through a one-pot three component reaction between phthalhydrazide or maleic hydrazide, Aryl aldehydes, and malononitrile in good to excellent yield (73–93%). The remarkable features of this approach include green catalyst, no organic solvent, metal-free, mild reaction, easy work-up and good functional group tolerance.

Synthesis of 1 H -Pyrazolo[1,2- b ]Phthalazine-5,10-Dione and 1 H -Pyrazolo[1,2- a ]Pyridazine-5,8-Dione Derivatives by Bovine Serum Albumin in Water