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An efficient synthesis of new 4-hydroxy-3-(6-aryl-2-(arylamino) pyrimidin-4-yl) quinolin-2(1 H )-ones  5a–j ,  6a–j , and the evaluation of their antimicrobial activity have been described. Potent antibacterial activity was shown by  6a, 5e, 5f, 6h, 6i, 5j  against the testes pathogenic bacteria. Also significant antifungal activity was shown by  6b, 6d, 5e  toward selective fungal strains.

Synthesis and In Vitro Antimicrobial Evaluation of New Quinolone Based 2-Arylamino Pyrimidines