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The aim of this study was to analyze the effect of the acute exposition of polycyclic aromatic hydrocarbons (PAHs) in four different concentrations (4.37, 8.73, 17.46, 34.95 µg L −1 ) in  Gambusia yucatana , an endemic fish of the Yucatán Peninsula by the characterization of the biomarkers activities: EROD, GST, TBARS, SOD, CAT, and GPx on liver; and AChE on brain. EROD and GST activities increased 292% and 754%, respectively, at the highest concentration ( p  &lt; 0.0001). TBARS decreased 51%, 49%, and 36% in the three last concentrations ( p  &lt; 0.0001). SOD activity increased 56% at the highest concentration ( p  &lt; 0.05). There was no difference in the GPx activity at any treatments. CAT activity decreased 85% at the highest concentration ( p  &lt; 0.005). AChE activity decreased at all treatments ( p  &lt; 0.0001). We concluded that PAHs are bioaccumulated and metabolized in  G. yucatana  mainly at a concentration of 34.95 µg L −1 ; SOD and GPx activities were affected during exposure period compared to the control, indicating the possible mechanism of oxidative stress and AChE could be a good indicator of exposure to PAHs. Finally, we believe that our results could be very useful for the development of  G. yucatana  conservation strategies.

Effects of Polycyclic Aromatic Hydrocarbons in Gambusia yucatana , an Endemic Fish from Yucatán Peninsula, Mexico