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Cynanotophyllosides E-F, two new minor pregnane glycosides were isolated from the antidepressant active fraction of cultivated  Cynanchum otophyllum , and their structures were determined as 12- O -vanilloyl-deacetylmetaplexigenin 3- O --D-glucopyranosyl-(1→4)--D-glucopyranosyl-(1→4)--D-cymaropyranosyl-(1→4)--D-oleandropyranosyl-(1→4)--D-digitoxopyranoside, and 12- O -nicotinoyl-deacetylmetaplexigenin 3- O --D-glucopyranosyl-(1→4)--D-glucopyranosyl-(1→4)--D-cymaropyranosyl-(1→4)--D-oleandropyranosyl-(1→4)--D-cymaropyranoside respectively, with the combination of spectroscopic and chemical analysis.

Cynanotophyllosides E-F, two minor pregnane glycosides from the roots of cultivated Cynanchum otophyllum