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A total of 16 fungal strains were isolated from fresh leaves and flowers of  Magnolia grandiflora  and the EtOAc extracts of them were assayed for antitumor activities. Among these, the fungus  Dothideomycetes  sp. BMC-101 with broad spectrum inhibition was selected for further study. Four alkaloids ( 1 – 4 ) including two new compounds (2-(hydroxyimino)-3-phenylpropanoyl)-L-phenylalanine ( 1 ) and 8-Acetyl-bisdethiobis(methylsulfanyl)apoaranotin ( 4 )) were isolated from  Dothideomycetes  sp. BMC-101. The structure of  1  was characterized with an oxime moiety formed by the condensation of two phenylalanines. To our knowledge, this is the first report on a fungal phenylalanine derivative with an oxime moiety.

Two new alkaloids produced by Dothideomycetes sp. BMC-101 isolated from the Magnolia grandiflora