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Chisocarpene A ( 1 ) is a new tirucallane-type triterpenoid together with odoratone ( 2 ) and 24-methylenecycloartanol ( 3 ), isolated from the stem bark of  Chisocheton lasiocarpus . The chemical structures of compounds  1–3  were elucidated through a detailed analysis of their spectroscopic data (IR, MS, 1 D, and 2 D NMR). The isolated compounds were evaluated for cytotoxic activity against the MCF-7 breast cancer cell line using a resazurin-based assay. Compound  1  showed the most potent activity (IC 50  26.56 ± 1.01 µM) and was two-fold more active than the positive control.

Tirucallane-type triterpenoid from the stem bark of Chisocheton lasiocarpus and its cytotoxic activity against MCF-7 breast cancer cells