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A new chromone analog ( 1 ) and a new pyrrole alkaloid ( 2 ), together with four known compounds, were isolated from the endophytic fungus  Penicillium sclerotiorum  MPT-250 obtained from the stems of  Taxus wallichiana  var.  chinensis  (Pilger) Florin. The structural elucidation of these metabolites was performed by high-resolution mass spectrometry and NMR spectroscopy. Compounds  1  and  5  exhibited significant antibacterial activity against carbapenems-resistant  Pseudomonas aeruginosa  and multidrug-resistant  Enterococcus faecium  with an minimum inhibitory concentration (MIC) value of 3.13 μg/ml respectively.

New chromone analog and pyrrole alkaloid produced by Penicillium sclerotiorum and their antibacterial activity