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Bioassay-guided separation afforded furanodienone 1,10-epoxide ( 9 ) as the new compound, curcolone ( 10)  as partially described compound and ten known compounds; germacrone ( 1 ), furanodienone ( 2) , curzerenone  (3 ), curcumenol ( 4 ), zederone ( 5 ), comosone II ( 6 ), (1E,4E,8R)-8-hydroxygermacra-1(10),4,7(11)-trieno-12,8-lactone ( 7 ), 13-hydroxygermacrone ( 8 ), curcuzederone ( 11 ) and demethoxycurcumin ( 12 ). The study showed that germacrone, furanodienone, curzerenone, comosone II, 13-hydroxygermacrone, curcuzederone and demethoxycurcumin are the bioactive compounds of  C. aeruginosa  rhizomes. Comosone II significantly inhibited MDA-MB-231 cell migration and invasion through the inhibition of MMP-9 enzyme. The present study may lead to further anticancer studies of comosone II and supports the traditional uses of  C. aeruginosa  rhizomes.

Bioactive compounds from Curcuma aeruginosa and the effect of comosone II on the migration and invasion of breast cancer cells