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Two new cyclic lipopeptides, acuminatums E ( 1 ) and F ( 2 ), together with four known cyclic lipopeptides, acuminatums A–D ( 3 – 6 ) were isolated from the corn culture of endophytic  Fusarium lateritium  HU0053. Their structures were elucidated by spectroscopic and advanced Marfey's amino acid analysis. All compounds were found to exhibit antifungal activities against  Penicillium digitatum . Acuminatum F ( 2 ), a new cyclic lipopeptide containing an unusual 3, 4-dihydroxy-phenylalanine unit exhibited the strongest antifungal activities with inhibition zone of 6.5 mm at the dose of 6.25 μg. Therefore, acuminatum F might be a potential environmental-friendly preservative for citrus fruits.

Acuminatums E and F, two new cyclic lipopeptides from Fusarium lateritium HU0053 and their antifungal activity