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The endophytic fungus  Diaporthe  sp. is known to contain many secondary metabolites, but fatty acid derivatives have rarely been found. In this study, four new fatty acid derivatives ( 1 – 4 ), together with four known compounds ( 5 – 8 ), were isolated from  Diaporthe  sp., which was obtained from the stem of  Ligularia fischeri . The absolute configurations of the new compounds  1 – 4  were deduced based on spectroscopic technique and  J -based coupling constant analysis. Moreover, compound  1  exhibited cytotoxic activities against HCT-8 and MCF-7 cancer cells, and compounds  3  and  4  showed modest selectivity for HCT-8 cells by MTT assay.

Four new fatty acid derivatives from Diaporthe sp. T24, an endophytic fungus isolated from Ligularia fischer