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Two novel acylated steroidal sapogenins, 3 β -acetoxy-1 α , 2 α , 4 β , 5 α , 7 α -pentahydroxy-spirost-25(27)-en-6-one ( 1 ) and (25S)-3 α -acetoxy-1 α , 2 β , 4 α , 5 α , 7 α -pentahydroxyspirostan-6-one ( 2 ), together with two known steroidal sapogenins ( 3  and  4 ), were isolated from  Rohdea chinensis  rhizomes. Their structures were elucidated by nuclear magnetic resonance (NMR) and mass spectrometry (MS) data. In addition, the antifungal activities of the isolated compounds against  Penicillium digitatum  and  Rhizopus stolonifera  were evaluated. Compound  2  significantly inhibited  R. stolonifera  growth, which was comparable to the positive control (sodium benzoate and carbendazim). Compound  4  showed the highest potency to inhibit  P. digitatum  growth compared to other compounds.

Two new steroidal sapogenins from Rohdea chinensis (synonym Tupistra chinensis ) rhizomes and their antifungal activity