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Stable isotope ratios of nitrogen and oxygen ( 15 N/ 14 N and  18 O/ 16 O) of nitrate (NO 3– ) are excellent tracers for developing systematic understanding of sources, conversions, and deposition of reactive atmospheric nitrogen (N r ) in the environment. Despite recent analytical advances, standardized sampling of NO 3– ) isotopes in precipitation is still lacking. To advance atmospheric studies on N r  species, we propose best-practice guidelines for accurate and precise sampling and analysis of NO 3–  isotopes in precipitation based on the experience obtained from an international research project coordinated by the International Atomic Energy Agency (IAEA). The precipitation sampling and preservation strategies yielded a good agreement between the NO 3–  concentrations measured at the laboratories of 16 countries and at the IAEA. Compared to conventional methods (e.g., bacterial denitrification), we confirmed the accurate performance of the lower cost Ti(III) reduction method for isotope analyses ( 15 N and  18 O) of NO 3–  in precipitation samples. These isotopic data depict different origins and oxidation pathways of inorganic nitrogen. This work emphasized the capability of NO 3–  isotopes to assess the origin and atmospheric oxidation of N r  and outlined a pathway to improve laboratory capability and expertise at a global scale. The incorporation of other isotopes like  17 O in N r  is recommended in future studies.

isotopes in environmental and health studies

Nitrate isotopes ( δ                  15 N,  δ                  18 O) in precipitation: best practices from an international coordinated research project