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Lipase B from  Candida antarctica  (CAL-B) was immobilised on epoxy-activated polymer support functionalised with polyethyleneimine (PEI) via preparation of enzyme aggregates by precipitation with polyethylene glycol (PEG 600). This involves adsorption of the aggregates on the functionalised polymer followed by cross-linking with glutaraldehyde. The apparent activity of the immobilised enzyme was found to be 750 TBU/g at optimum pH 7.5 and temperature 30 °C. The immobilisation yield was 24%. The immobilised enzyme was used for desymmetrization of  cis -3,5-diacetoxy-1-cyclopentene in aqueous buffer (70% yield, &gt;99%  e.e. ) and methyl  tert -butyl ether (95% yield and &gt; 99%  e.e ) to obtain (1  R ,4 S )-4-hydroxycyclopent-2-en-1-yl acetate. The immobilised enzyme could be recycled (5 times) in both aqueous buffer and organic solvents. Further, the expensive polymer support was also recycled (5 times) after the immobilised enzyme lost its activity by washing with 1 N HCl.

biocatalysis and biotransformation

Polymer supported cross-linked enzyme aggregates (CLEAs) of lipase B from  Candida antarctica : An efficient and recyclable biocatalyst for reactions in both aqueous and organic media