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Males and females of various species spend energy on different activities throughout the day in their natural environment. Thus, the present study aimed to assess whether males and females of  A. nyassae  differ in learning and preferences through two experiments. Experiment 1 subjected animals to learning by association in a T-maze test. Experiment 2 subjected males and females to a preference test in a T-maze, with each tank arm receiving one of the two following positive stimuli: shelter or food. Experiment 1 found that both sexes were able to learn the association between neutral and positive stimuli. Experiment 2 found that, regardless of sex, the animals frequented more the arm with the presence of shelter. This study demonstrates that males and females of  A. nyassae  were able to learn the task of object and food association and also preferred to take shelter over eating, even after exposure to stress.

Association learning and preference of females and males of the blue orchid, Aulonocara nyassae Regan, 1922 in a T-maze