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The paper reports that the main constituent of essential oil from the leaves of  Macclurodendron oligophlebia  (Merr.) Hartl. growing in Vietnam was α-pinene (81.7%), while α-pinene (67.4%) and limonene (11.2%) were the major compounds in fruit essential oil. The composition of essential oils from the leaves and fruits of  Melicope ptelefolia  (Champ. ex Benth.) T.G. Hartley was characterized as germacrene D (34.0% and 31.1%, respectively), bicyclogermacrene (5.5% and 7.6%, respectively), d-elemene (9.9% and 9.0%, respectively) and α-pinene (6.5% and 9.8%, respectively). The fruit essential oil contains limonene (4.9%) and ( E )-β-ocimene (8.1%). The fruit essential oils of  M. oligophlebia  and  M. ptelefolia  exhibited anti-candial activity towards  Candida albicans  ATCC 10231, with minimum inhibitory concentration (MIC) value of 8.0 μg/mL and 32.0 μg/mL, respectively.  M. oligophlebia  fruit oil also showed action against  Enterococcus faecalis  ATCC 29212,  Staphylococcus aureus  ATCC 25923, and  Bacillus cereus  ATCC 14579, each with MIC value of 32.0 μg/mL. Also,  M. oligophlebia  leaf showed higher activity against  Pseudomonas aeruginosa  ATCC 27853 (MIC, 32.0 μg/mL). The other oil samples exhibited varying degrees of antimicrobial activity with MIC values of either 64.0 μg/mL or 128. 0 μg/mL. The leaf essential oil of  M. oligophlebia  displayed larvicidal activity against  Aedes aegypti  larvae with minimum lethal concentration (LC 50 ) values of 16.71 μg/mL and 11.40 μg/mL, respectively at 24 h and 48 h. The fruit oil did not exhibit any larvicidal action. However,  M. ptelefolia  leaf and fruit showed activity at 24 h with LC 50  values of 33.18 μg/mL and 35.06 μg/mL, respectively.

journal of essential oil bearing plants

Chemical compositions, antimicrobial activity and mosquito larvicidal actions of essential oils from the leaves and fruits of two Rutaceae family of plants from Vietnam