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This paper investigated the chemical composition of the essential oil (EO) of  Origanum floribundum  Munby. by gas chromatography coupled with mass spectrometry and evaluated its antimicrobial activity. The compositional analysis identified 33 compounds representing 97.26% of the total composition, of which the majority component was carvacrol at 41.092%. The antimicrobial activity evaluated deduced that  O. floribundum  EO affects all tested strains, of which the most susceptible were  Staphylococcus aureus  and  Candida albicans , with growth inhibition zones of 25.83 mm and 23.25 mm, respectively. A bactericidal effect was recorded on  Staphylococcus aureus  and  Bacillus cereus  at 1.56 mg/mL and 3.125 mg/mL EO, respectively, and a fungistatic effect on  Candida albicans  at 1.56 mg/mL of EO. The intestinal microbiota strains,  Bifidobacterium animalis  subsp  lactis  and  Lactobacillus rhamnosus LbRE-LSAS , were not affected by  O. floribundum  EO.

Chemical composition and antimicrobial activity of the essential oil of oregano ( Origanum floribundum Munby.) endemic to northern Algeria