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The paper reports the chemical compositions and antimicrobial activity of essential oils from  Blumea lacera  (Burm. f.) DC.,  Tridax procumbens  (L.) L., and  Ageratum houstonianum  Mill., of Asteraceae family grown in Vietnam. The essential oils were isolated by hydrodisitllation and analysed by GC and GC/MS. The Minimum inhibitory concentration (MIC) and Median inhibitory concentration (IC 50 ) values were measured by the microdilution broth susceptibility assay. The main compounds of  B. lacera  were germacrene D (25.5%), 2,5-dimethoxy- p -cymene (20.6%), β-caryophyllene (17.9%), thymol methyl ether (6.3%).  T. procumbens  afforded essential oil whose major constituent was β-pinene (13.4%), while germacrene D (21.3%), α-pinene (19.0%), and β-caryophyllene (8.9%) constitute the bulk of  A. houstenianum  essential oil. The leaf essential oils of  A. houstenianum  collected in August (MIC, 8.0 μg/mL) and  B. lacera  (MIC, 16.0 μg/mL) were the most active against  Enterococcus faecalis  ATCC 29212, with corresponding IC 50  values of 4.00 μg/mL and 5.67 μg/mL, respectively. The essential oil of  T. procumbens  exhibited the most potent activity towards  Staphylococcus aureus  ATCC 25923 and  Bacillus cereus  ATCC 14579, with MIC value of 32.0 μg/mL and IC 50  values of 12.45 μg/mL and 7.89 μg/mL, respectively. Also, both  A. houstenianum  essential oils collected in August and April displayed antibacterial action against  Pseudomonas aeruginosa  ATCC 27853 with MIC values of of 64.0 μg/mL (IC 50 , 21.45 μg/mL) and 32.0 μg/mL and (IC 50 , 15.22 μg/mL). Both  B. lacera  and  T. procumbens  showed anti-candidal property with MIC value of 16.0 μg/mL, as well as IC 50  values of 5.69 μg/mL and 8.66 μg/mL, respectively.

journal of essential oil bearing plants

Chemical Compositions of Essential Oils and Antimicrobial Activities of Asteraceae: The Leaves of  Blumea lacera  (Burm. f.) DC.,  Tridax procumbens  (L.) L., and  Ageratum houstonianum  Mill., from Vietnam