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The essential oils from the stem of  Etlingera velutina  was obtained by a Clevenger-apparatus and the chemical composition of the volatile oil was identified using GC-FID and GC-MS methods. the oil consists of sesquiterpene hydrocarbon. α-Cubebene (29.98%) and 2-undecanone (20.04%) were the main components for the rhizome oil. In the cellular and molecular part of this study, the MTT assay was run to treat of the cell lines by EO for evaluation of the cytotoxicity and anti-human gastric cancer properties of EO. The results revealed a dose-dependently on the viability of malignant gastric cell line. The IC 50  of EO were 376 and 371 μg/mL against NCI-N87 and MKN45 cell lines, respectively. For antioxidant analysis, the IC 50  of 192 μg/mL was obtained for radical scavenging activity of EO against DPPH.

Introducing a Modern Chemotherapeutic Drug Formulated by Etlingera velutina Essential Oil for the Treatment of Human Gastric Cancer