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Citrus  genus is notable for peel essential oils which are valuable by-products during  Citrus  processing. In this study, it was aimed to determine chemical composition and antimicrobial activity of the essential oil of pummelo peel (PEO). The essential oil was obtained by hydrodistillation from flavedo layer of the peel. It was subjected to Gas Chromatography/Mass Spectrometry (GC/MS) for identification of its volatiles and a total of sixty-two compounds were identified. Monoterpenes constituted 89.36% of PEO, mostly by D-limonene (76.97%) and β-myrcene (6.37%). The essential oil became prominent especially with its nootkatone content (1.13%). In antimicrobial activity assays, broth macrodilution method was used against to  Staphylococcus aureus, Bacillus cereus, Escherichia coli ,  Salmonella  Enteritidis,  Saccharomyces cerevisiae  and  Candida albicans . According to the test results, minimum inhibitory concentrations of the essential oil ranged between 2000 and 16000 µg/mL for  S. aureus, B. cereus, S. cerevisiae  and  C. albicans . No inhibitory effect was observed on  E. coli  and  S.  Enteritidis although 32000 µg/mL PEO was applied. On the basis of the results obtained, it was thought that PEO could be used as a natural and inexpensive flavouring and antimicrobial agent by the food and pharmaceutical industries via valorization of pummelo peel.

Chemical Composition and Antimicrobial Activity of Pummelo ( Citrus maxima ) Essential Oil Derived from Fruit Peel