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In the present study, 15 fungal isolates were found. The antagonistic potential of seven of these, including  Trichoderma harzianum ,  T. viride ,  T. virens ,  Aspergillus fumigatus ,  A. niger ,  Aspergillus  sp., and  Drechslera triseptata  were proved under  in vitro  and greenhouse conditions. In the dual culture method,  T. virens  and  T. viride  (65.51%), in the volatile compounds method,  T. harzianum  (66.66%), and in the non-volatile compounds method,  T. virens  (63%), had the highest inhibitory effect on fungal mycelial growth. In the slide culture method, all the studied isolates prevented the progress of pathogenic fungal hyphae and in some deformed the hyphae. All the inoculated fungi reduced the disease severity of  B. cinerea , among which  T. virens  was the most effective fungus, reducing the disease severity by 26%. Also, the examination of height, fresh weight, and dry weight of rose plants under greenhouse conditions revealed that the application of  T. virens  increased these characteristics in the presence of the pathogen. The application of other fungi tested also increased these characteristics in the presence of  B. cinerea .

Biological control of Botrytis cinerea, the causal agent of rose gray mold disease by antagonistic fungi