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Strain NB-1, which can efficiently degrade nitrobenzene, was identified as  Pseudomonas frederiksbergensis . NB-1 was resistant to cold and alkali with the widest temperature (4–35 °C) and pH (5–11) adaptive range, compared with other reported nitrobenzene-degrading microorganisms. Based on the Haldane-Andrews model, the real maximum specific growth rate  μm ', specific affinity a A , and inhibition coefficient K i  were used in response surface methodology (RSM) simultaneously for the first time to guide NB-1 to treat nitrobenzene wastewater. According to the RSM model, the environmental factors (temperature, pH, salinity) corresponding to the optimal values of  μm ', a A , and K i  were determined. By comparing the specific growth rates corresponding to the optimal values of  μm ', a A , and K i , respectively, the optimum growth conditions of NB-1 were determined under different nitrobenzene concentrations. The study of  μm ', a A , and K i  by RSM provided a new approach for a more accurate optimization of biological wastewater treatment conditions.

environmental technology

Effects of temperature, pH, and salinity on the growth kinetics of  Pseudomonas  sp. NB-1, a newly isolated cold-tolerant, alkali-resistant, and high-efficiency nitrobenzene-degrading bacterium