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An electrochemiluminescence (ECL) aptasensor was prepared to detect Pb 2+  with nitrogen-doped carbon quantum dots (NCQDs) as ECL materials. To prepare the working electrode, NCQDs with carboxyl groups were loaded on a glassy carbon electrode (GCE) and then Pb 2+  aptamers were covalently bound to the NCQDs to form a stable GCE/NCQDs/aptamers. On addition of Pb 2+ , the chain aptamers change to a pb 2+  G-quadruplex conformation, which lead to a large decrease in the ECL intensity. The variation of intensity and the logarithm of the Pb 2+  concentration had a good linear relationship ( R2  = 0.998). The detection range was wide (50 pM to 387.9 nM) with a low detection limit (18.9 pM). In interference experiments, the ECL Pb 2+  aptasensor did not suffer from interference and it had good stability. The NCQDs ECL aptasensor can detect Pb 2+  quickly and accurately, and provides a fast and efficient method for detection of Pb 2+ . Compared with literatures, the Pb 2+  aptasensor has simpler preparation process, lower cost; furthermore, it is more environmentally friendly.

A simple electrochemiluminesecence aptasenor using a GCE/NCQDs/aptamers for detection of Pb