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To combat dandruff associated pathogens, supercritical fluid extraction (SFE) can be used as an alternative eco-friendly technique to obtain antimicrobial plant extracts over conventional methods. The purpose of the study was to compare the yield and antimicrobial potential of extracts obtained by different extraction methods. Extraction was carried out by cold percolation method using ethyl acetate (EA) and SFE using CO 2 . Antimicrobial activity was studied against dandruff causing microbes;  Malassezia furfur ,  Propionibacterium acne,  and  Staphylococcus epidermidis  by agar well diffusion and micro broth dilution method. Statistical evaluation was done by principal component analysis (PCA). The yield was found higher in the ethyl acetate extracts. PCA indicated that among the sixteen extracts, SFE extract of  Azadirachta indica  leaves was the most influential with the highest F1 score and maximum antimicrobial activity.  Cinnamomum zeylanicum  SFE extract demonstrated the lowest MIC against  P. acne  and  M. furfur.  GC-MS analysis of  A. indica  extract inferred that ganoderic acid, 13,14-epoxyoleanan-3-ol acetate, henicosanal, 2-heptadecycloxirane were the major phytoconstituents whereas cinnamaldehyde, α- muurolene and caffeic acid were primarily found in cinnamon. Bioactive compounds identified in the extracts of  A. indica  and  C. zeylanicum  can be used in natural antidandruff products.

journal of dermatological treatment

Comparative antidandruff efficacy of plant extracts prepared from conventional and supercritical fluid extraction method and chemical profiling using GCMS