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Laser speckle contrast imaging (LSCI) is a real-time, full-field, non-contact optical imaging technique that has been widely used in blood flow imaging. However, its practical applications are limited due to its low signal-to-noise ratio (SNR) and low image contrast. In this study, a fusion denoising algorithm based on homomorphic transform and block-matching and 3D transform-domain collaborative filtering algorithm is proposed to reduce noise and improve image contrast. The reliability and applicability of the proposed method were evaluated by a phantom experiment and two  in vivo  experiments. The results show that the proposed algorithm can effectively remove the noise and enhance the image contrast. Consequently, the SNR is increased, the dynamic range of LSCI is expanded, and the estimation accuracy of blood flow is improved.

A fusion denoising method based on homomorphic transform and 3D transform-domain collaborative filtering for laser speckle imaging of blood flow