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Carbon dots (CDs) are emerging as new promising fluorescence probes for stimulated emission depletion microscopy, according to the excellent biocompatibility, low saturation intensity and broad excitation spectra to be compatible for different wavelength combinations. In this study, three kinds of CDs synthesized with phenylenediamines using a facile solvothermal approach have been characterized in detail. The influence of various organic solvents on suppression factors () is investigated. The results indicate that the curves of suppression factors in alcohol solvent are generally in linear relation with depletion power, rather than the conventional reciprocal relation. Moreover, CDs of 15 nm diameter display an ultralow saturation intensity ∼0.4 MW/cm 2  in alcohol solvent at 492/10 nm fluorescence band, which is one-order improvement compared with common ATTO fluorescence dyes. The results indicate CDs have shown great promise as potential nanoprobes for stimulated emission depletion imaging.

Depletion of carbon dots in stimulated emission depletion microscopy developed with 405/532 nm continuous-wave lasers