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The current study aims to describe the functional changes in Murrah buffalo PBMCs during chronic in-vitro thermal stress. Thus, PBMCs were stimulated at 34°C or 37°C or 40°C for 0 hr, 24 hrs and 48 hrs. At the end of each treatment, mRNA abundance of genes related to heat shock (HSP10, HSP40, HSP60, HSP70, HSP90 and HSF1), inflammation (IL1β, IL6, IL 12 and INF γ), cell viability (Bax and Bcl2) and natural resistance-associated macrophage protein (SLC11A1) were quantified by qPCR. The results revealed upregulation (p &lt; 0.05) in mRNA expression of HSP10, HSP40, HSP60, HSP70, HSF1, IL1β, IL6, IL12, INF γ, BAX, BCL2 and SLC11A1 gene in chronically (24 and 48 hrs) thermal stressed (40 °C) PBMCs than cells maintained at 34 °C or 37 °C. In conclusion, chronic thermal stress directly affects the functionality of Murrah buffalo PBMCs and is likely responsible for immune alterations during thermal stress.

Chronic in vitro thermal stress alters functional response of peripheral blood mononuclear cells of Murrah buffalo