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In this study, effect high-energy diet with supplemented with copper and zinc on oxidative stress, leukocyte function and reproductive performance during the transition period in the crossbred cow under tropical settings. Advanced pregnant crossbred cows of two to four parity and lactation yield of &gt;10 L/day were divided into three equal groups ( n  = 10 cow/group). Group I cows were fed basal diet to serve as control. Group II cows were supplemented with 20% additional concentrate, copper (15.7/kg DM) and zinc (22 mg/kg DM). Group III cows were provided with only energy in the form of 20% additional concentrate. Copper and zinc were supplemented from −4 before to +8 week of calving, whereas concentrate was given from +2 to +8 week after calving. Blood samples were collected at weekly interval from −4 to +8 week of calving. Group II had a significantly lower MDA, higher TAC and decreased activity of SOD and CAT than that of Group I ( P  &lt; 0.05). Phagocytic activity and lymphocytic activity increased from 3 to 8 weeks postpartum ( P  &lt; 0.05) in Group II and III indicating early restoration of leukocyte function. Resumption of postpartum oestrus was shorter in group II ( P  &lt; 0.05).

biological rhythm research

Effect of high-energy diet supplemented with additional copper and zinc during the transition period on oxidative stress and postpartum reproductive performance in the crossbred cows